Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE.

Techniques: Virus, Plasmid Preparation, shRNA, Recombinant, DNA Labeling, Staining, Sequencing, Software, Imaging, Light Microscopy

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Plasmid Preparation, shRNA, Recombinant, DNA Labeling, Staining, Sequencing, Software, Imaging, Light Microscopy

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Plasmid Preparation, shRNA, Recombinant, DNA Labeling, Staining, Sequencing, Software, Imaging, Light Microscopy

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: (A) Co-staining of ALDH1A1 and TH in midbrain coronal sections of Aldh1a1-P2A-CreERT2 mice after injection with mCherry or taCasp3-expressing AAVs for three weeks. The arrow indicates the remaining ALDH1A1+ mDANs in the VTA regions. Scale bar: 500 μm.

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Staining, Injection, Expressing

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: (A and B) Quantifications of the distance (A) (unpaired t test, two-tailed, mCherry+saline, n = 1M, 3F, versus mCherry+L-DOPA, n = 1M, 3F, p = 0.65; taCasp3+saline, n = 4M, 3F, versus taCasp3+L-DOPA, n = 5M, 2F, p = 0.0066), and velocity distribution of control and ALDH1A1+ nDAN-ablated mice treated with L-DOPA or saline in open-field tests (B). Arrows indicates the divergence at a speed > 15 cm/s. Wilcoxon matched-pairs signed rank test, p < 0.0001.

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Two Tailed Test

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Plasmid Preparation, shRNA, Recombinant, DNA Labeling, Staining, Sequencing, Software, Imaging, Light Microscopy

Journal: Cell reports

Article Title: Distinct Connectivity and Functionality of Aldehyde Dehydrogenase 1a1-Positive Nigrostriatal Dopaminergic Neurons in Motor Learning

doi: 10.1016/j.celrep.2019.06.095

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cg-Gt(ROSA)26Sor tm9(CAG-tdTomato)Hze /J The Jackson Laboratory Cat# 007909 RRID: IMSR_JAX:007909 Mouse: C57BL/6J The Jackson Laboratory Cat# 000664; RRID: IMSR_JAX:000664 Oligonucleotides P2A sequence: 5′-ggatccggagccacgaacttctctctgttaaagcaagcaggagacgtggaagaaaaccccggtcct-3′ Szymczak-Workman et al., 2012 N/A Primers for 5′ Southern Probe 5′-Pb-F: 5′-agagttcaacagccccataaaa-3′ This paper Custom; N/A Primers for 5′ Southern Probe 5′-Pb-R: 5′-gtgtgccatgtcaagaagtgtt-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-F: 5′-gatggcaagcttcctcataatc-3′ This paper Custom; N/A Primers for 3′ Southern Probe 3′-Pb-R: 5′-tacagggttccatgagtgagtg-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-F: 5′-cttcaaggagtcactgagggac-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-Cre-F: 5′-gcgcgcctgaagatatagaaga-3′ This paper Custom; N/A Genotyping primers for Aldh1a1 +/P2A-CreERT2 mice Ald1-wt-R: 5′-gctcaagacagtcgcaatgaag-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-F: 5′-agtagctacaatggaaagcacct-3′ This paper Custom; N/A Genotyping primers for Neo-Frt deletion Ald1-delNeo-R: 5′-acacatctcctatagtaaccagca-3′ This paper Custom; N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-F: 5′-gctgtggtggctactttcactatgc-3′ UCDAVIS KOMP Repository N/A Genotyping primers for Dat +/fl ( Slc6a3 +/fl ) mice CSD-Slc6a3-ttR: 5′-aacctgaagaaggtcagcactaccc-3′ UCDAVIS KOMP Repository N/A Recombinant DNA Plasmid: pDNDF-7 vector A gift from Hoon Shim N/A Plasmid: pBlueScript II SK (+) Aglient Technologies Cat# 212205 Plasmid: pCAG-CreERT2 A gift from Connie Cepko Addgene Plasmid #14797 Plasmid: pAAV-CAG-Flex-TCB A gift from Liqun Luo Addgene Plasmid #48332 Plasmid: pAAV-CAG-Flex-RG A gift from Liqun Luo Addgene Plasmid #48333 Plasmid: pAAV-EF1a-Flex-taCasp3-TEVp A gift from Nirao Shah & Jim Wells Addgene Plasmid #45580 Plasmid: pAAV-Aldh1a1 shRNA/EGFP A gift from Lu Chen N/A Software and Algorithms Neurolucida MBF Bioscience RRID:SCR_001775 Stereo Investigator MBF Bioscience RRID:SCR_002526 Prism 7 GraphPad RRID:SCR_002798 ZEN Digital Imaging for Light Microscopy Zeiss RRID:SCR_013672 Stereo Investigator MBF Bioscience RRID:SCR_002526 MATLAB Script for Open-field test Barbera et al., 2016 N/A Open in a separate window KEY RESOURCES TABLE Whole-brain connectivity map of ALDH1A1 + nigrostriatal dopaminergic neurons (nDANs) Reciprocal innervations between ALDH1A1 + nDANs and dorsal striatal neurons ALDH1A1 + nDANs are essential for rotarod motor skill acquisition Dopamine therapy fails to restore motor learning in the ALDH1A1 + nDAN-ablated mice

Techniques: Plasmid Preparation, shRNA, Recombinant, DNA Labeling, Staining, Sequencing, Software, Imaging, Light Microscopy